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Saitoh Shigeaki
Division of Cell Biology Institute of Life Science
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分裂酵母（S.pombe）や哺乳類培養細胞をモデル生物として利用し、染色体分配機構や細胞周期制御メカニズムの研究を行っています。進化的に保存された動原体構成タンパク質Mis6の機能解析で学位を取得した後（指導教授：柳田充弘教授 京都大学大学院理学研究科）、米国カリフォルニア州スクリプス研究所に留学し、P.Russell博士の元で細胞周期チェックポイントの研究を行いました。その過程でCid13と名付けたタンパク質が、進化的に保存された細胞質ポリAポリメラーゼであることを突き止めました。帰国後は久留米大学分子生命科学研究所に所属し、引き続き染色体分配・細胞周期コントロール機構の研究を行っています。現在は、栄養飢餓とそれらのコントロール機構との関連性に興味を持っています。また、研究の過程で培った技術や成果の産業応用を視野に入れながら、国内外の企業や研究者との共同研究も進めています。
Research activity information
■ Paper
■ Research Themes
- S. pombe Mis4 is required for exit from G0 as it is necessary for full nuclear separation during the subsequent M phase
Michiko Suma; Orie Arakawa; Yuria Tahara; Kenichi Sajiki; Shigeaki Saitoh; Mitsuhiro Yanagida
Journal of Cell Science, 01 Mar. 2025, [Reviewed]
Corresponding - Arrayed CRISPRi library to suppress genes required for Schizosaccharomyces pombe viability
Ken Ishikawa; Saeko Soejima; Takashi Nishimura; Shigeaki Saitoh
Journal of Cell Biology, 06 Jan. 2025, [Reviewed]
Corresponding - Proliferation Analyses of Conditional Knockdown Strains Using CRISPR Interference in Fission Yeast
Ken Ishikawa; Shigeaki Saitoh
Methods in Molecular Biology, 12 Nov. 2024 - Phosphorylation of Aly3 C-terminus impedes aberrant endocytosis ofS. pombehexose transporter Ght5
Yusuke Toyoda; Fumie Masuda; Shigeaki Saitoh
27 Mar. 2024 - In fission yeast, 65 non-essential mitochondrial proteins related to respiration and stress become essential in low-glucose conditions
Ayaka Mori; Lisa Uehara; Yusuke Toyoda; Fumie Masuda; Saeko Soejima; Shigeaki Saitoh; Mitsuhiro Yanagida
Royal Society Open Science, 18 Oct. 2023, [Reviewed]
Corresponding - Genetic knockdown of genes that are obscure, conserved and essential using CRISPR interference methods in the fission yeast S. pombe
Ken Ishikawa; Saeko Soejima; Shigeaki Saitoh
Journal of Cell Science, 01 May 2023, [Reviewed]
Corresponding - Transcriptional Regulation Technology for Gene Perturbation in Fission Yeast
Ken Ishikawa; Shigeaki Saitoh
Biomolecules, 21 Apr. 2023, [Reviewed], [Invited] - Growth conditions inducing G1 cell cycle arrest enhance lipid production in the oleaginous yeast Lipomyces starkeyi.
Yasutaka Morimoto; Shigeaki Saitoh; Yuko Takayama
Journal of cell science, 14 Jul. 2022, [Reviewed] - Characterization of hexose transporter genes in the views of the chronological life span and glucose uptake in fission yeast
Teppei Maruyama; Kanako Hayashi; Kotaro Matsui; Yasukichi Maekawa; Takafumi Shimasaki; Hokuto Ohtsuka; Shigeaki Saitoh; Hirofumi Aiba
The Journal of General and Applied Microbiology, 2022, [Reviewed] - Fission Yeast TORC2 Signaling Pathway Ensures Cell Proliferation under Glucose-Limited, Nitrogen-Replete Conditions
Yusuke Toyoda; Shigeaki Saitoh
Biomolecules, 06 Oct. 2021, [Reviewed], [Invited]
Corresponding - TORC2 inhibition of α-arrestin Aly3 mediates cell surface persistence of S. pombe Ght5 glucose transporter in low glucose
Yusuke Toyoda; Saeko Soejima; Fumie Masuda; Shigeaki Saitoh
Journal of Cell Science, 15 May 2021, [Reviewed]
Corresponding - Implementation of dCas9-mediated CRISPRi in the fission yeast Schizosaccharomyces pombe
Ken Ishikawa; Saeko Soejima; Fumie Masuda; Shigeaki Saitoh
G3 Genes|Genomes|Genetics, 15 Apr. 2021, [Reviewed]
Corresponding - Multiple nutritional phenotypes of fission yeast mutants defective in genes encoding essential mitochondrial proteins
Lisa Uehara; Shigeaki Saitoh; Ayaka Mori; Kenichi Sajiki; Yusuke Toyoda; Fumie Masuda; Saeko Soejima; Yuria Tahara; Mitsuhiro Yanagida
Open Biology, Apr. 2021, [Reviewed]
Lead, Corresponding - Identification of 15 New Bypassable Essential Genes of Fission Yeast
Aoi Takeda; Shigeaki Saitoh; Hiroyuki Ohkura; Kenneth E. Sawin; Gohta Goshima
Cell Structure and Function, 2019, [Reviewed] - Extracellular glucose level regulates dependence on GRP78 for cell surface localization of multipass transmembrane proteins in HeLa cells
Yusuke Toyoda; Busra Akarlar; Mihail Sarov; Nurhan Ozlu; Shigeaki Saitoh
FEBS Letters, Oct. 2018, [Reviewed]
Corresponding - Fission yeast ceramide ts mutants cwh43 exhibit defects in G0 quiescence, nutrient metabolism, and lipid homeostasis
Norihiko Nakazawa; Takayuki Teruya; Kenichi Sajiki; Kazuki Kumada; Alejandro Villar-Briones; Orie Arakawa; Junko Takada; Shigeaki Saitoh; Mitsuhiro Yanagida
Journal of Cell Science, 01 Jan. 2018, [Reviewed] - Glucose restriction induces transient G2 cell cycle arrest extending cellular chronological lifespan
Fumie Masuda; Mahiro Ishii; Ayaka Mori; Lisa Uehara; Mitsuhiro Yanagida; Kojiro Takeda; Shigeaki Saitoh
Scientific Reports, Apr. 2016, [Reviewed]
Corresponding - Adaptive regulation of glucose transport, glycolysis and respiration for cell proliferation.
Shigeaki Saitoh
Biomolecular concepts, 01 Dec. 2015, [Reviewed], [Invited]
Corresponding - Mechanisms of expression and translocation of major fission yeast glucose transporters regulated by CaMKK/phosphatases, nuclear shuttling, and TOR
Shigeaki Saitoh; Ayaka Mori; Lisa Uehara; Fumie Masuda; Saeko Soejima; Mitsuhiro Yanagida
Molecular Biology of the Cell, 15 Jan. 2015, [Reviewed]
Lead, Corresponding - Does a shift to limited glucose activate checkpoint control in fission yeast?
Shigeaki Saitoh; Mitsuhiro Yanagida
FEBS Letters, 01 Aug. 2014, [Reviewed], [Invited]
Lead - Switching the centromeres on and off: epigenetic chromatin alterations provide plasticity in centromere activity stabilizing aberrant dicentric chromosomes
Hiroshi Sato; Shigeaki Saitoh
Biochemical Society Transactions, 01 Dec. 2013, [Reviewed], [Invited]
Corresponding - Epigenetic Inactivation and Subsequent Heterochromatinization of a Centromere Stabilize Dicentric Chromosomes
Hiroshi Sato; Fumie Masuda; Yuko Takayama; Kohta Takahashi; Shigeaki Saitoh
Current Biology, Apr. 2012, [Reviewed]
Corresponding - Specific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast
Tomáš Pluskal; Takeshi Hayashi; Shigeaki Saitoh; Asuka Fujisawa; Mitsuhiro Yanagida
FEBS Journal, Apr. 2011, [Reviewed] - CENP-A Reduction Induces a p53-Dependent Cellular Senescence Response To Protect Cells from Executing Defective Mitoses
Kayoko Maehara; Kohta Takahashi; Shigeaki Saitoh
Molecular and Cellular Biology, May 2010, [Reviewed]
Last - Hsk1- and SCFPof3-Dependent Proteolysis of S. pombe Ams2 Ensures Histone Homeostasis and Centromere Function
Yuko Takayama; Yasmine M. Mamnun; Michelle Trickey; Susheela Dhut; Fumie Masuda; Hiroyuki Yamano; Takashi Toda; Shigeaki Saitoh
Developmental Cell, Mar. 2010, [Reviewed]
Last - Dual Regulation of Mad2 Localization on Kinetochores by Bub1 and Dam1/DASH that Ensure Proper Spindle Interaction
Shigeaki Saitoh; Yasuyo Kobayashi; Yuki Ogiyama; Kohta Takahashi
Molecular Biology of the Cell, Sep. 2008, [Reviewed]
Lead, Corresponding - Expression of a novel 90-kDa protein, Lsd90, involved in the metabolism of very long-chain fatty acid-containing phospholipids in a mitosis-defective fission yeast mutant
Kazuaki Yokoyama; Miyuki Nakagawa; Masayuki Satoh; Shigeaki Saitoh; Naoshi Dohmae; Ayako Harada; Noriko Satoh; Ken Karasawa; Koji Takio; Mitsuhiro Yanagida; Keizo Inoue
JOURNAL OF BIOCHEMISTRY, Mar. 2008, [Reviewed] - Biphasic Incorporation of Centromeric Histone CENP-A in Fission Yeast
Yuko Takayama; Hiroshi Sato; Shigeaki Saitoh; Yuki Ogiyama; Fumie Masuda; Kohta Takahashi
Molecular Biology of the Cell, Feb. 2008, [Reviewed] - The fission yeast DASH complex is essential for satisfying the spindle assembly checkpoint induced by defects in the inner-kinetochore proteins
Yasuyo Kobayashi; Shigeaki Saitoh; Yuki Ogiyama; Saeko Soejima; Kohta Takahashi
Genes to Cells, Mar. 2007, [Reviewed] - Spindle Checkpoint Signaling Requires the Mis6 Kinetochore Subcomplex, Which Interacts with Mad2 and Mitotic Spindles
Shigeaki Saitoh; Kojiro Ishii; Yasuyo Kobayashi; Kohta Takahashi
Molecular Biology of the Cell, Aug. 2005, [Reviewed]
Lead - Two distinct pathways responsible for the loading of CENP-A to centromeres in the fission yeast cell cycle
Kohta Takahashi; Yuko Takayama; Fumie Masuda; Yasuyo Kobayashi; Shigeaki Saitoh
Philosophical Transactions of the Royal Society B: Biological Sciences, 29 Mar. 2005 - A cell cycle-regulated GATA factor promotes centromeric localization of CENP-A in fission yeast
ES Chen; S Saitoh; M Yanagida; K Takahashi
MOLECULAR CELL, Jan. 2003, [Reviewed] - Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA
S Saitoh; A Chabes; WH McDonald; L Thelander; Yates, JR; P Russell
CELL, May 2002, [Reviewed]
Lead - Very-long-chain fatty acid-containing phospholipids accumulate in fatty acid synthase temperature-sensitive mutant strains of the fission yeast Schizosaccharomyces pombe fas2/lsd1
Kazuaki Yokoyama; Shigeaki Saitoh; Mayuko Ishida; Yoshio Yamakawa; Kazumi Nakamura; Keizo Inoue; Ryo Taguchi; Akira Tokumura; Masahiro Nishijima; Mitsuhiro Yanagida; Morio Setaka
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 29 Jun. 2001 - Application of the chromatin immunoprecipitation method to identify in vivo protein-DNA association in fission yeast.
2000 - Proper metaphase spindle length is determined by centromere proteins Mis12 and Mis6 required for faithful chromosome segregation
G Goshima; S Saitoh; M Yanagida
GENES & DEVELOPMENT, Jul. 1999, [Reviewed] - Mis6, a fission yeast inner centromere protein, acts during G1/S and forms specialized chromatin required for equal segregation
S Saitoh; K Takahashi; M Yanagida
CELL, Jul. 1997, [Reviewed]
Lead - Use of green fluorescent protein for intracellular protein localization in living fission yeast cells
K Nabeshima; S Saitoh; M Yanagida
CELL CYCLE CONTROL, 1997 - Aberrant mitosis in fission yeast mutants defective in fatty acid synthetase and acetyl CoA carboxylase
S Saitoh; K Takahashi; K Nabeshima; Y Yamashita; Y Nakaseko; A Hirata; M Yanagida
JOURNAL OF CELL BIOLOGY, Aug. 1996, [Reviewed]
Lead - FISSION YEAST CUT3 AND CUT14, MEMBERS OF A UBIQUITOUS PROTEIN FAMILY, ARE REQUIRED FOR CHROMOSOME CONDENSATION AND SEGREGATION IN MITOSIS
Y SAKA; T SUTANI; Y YAMASHITA; S SAITOH; M TAKEUCHI; Y NAKASEKO; M YANAGIDA
EMBO JOURNAL, Oct. 1994, [Reviewed]
- AID, which is ectopically expressed in colonic epithelial cells under inflammatory conditions, regulates target gene expression by inducing DNA demethylation.
岡田季之; 豊田雄介; 溝口充志; 齋藤成昭
日本分子生物学会年会プログラム・要旨集(Web), 2023
■ Research Themes
- Production of a valuable polyunsaturated fatty acid by CRISPR-mediated metabolic engineering
Aug. 2024 - Oct. 2024 - genetic analysis of cellular adaptation to glucose starvation
Grant-in-Aid for Scientific Research (C)
Kurume University
Apr. 2017 - Mar. 2020 - Molecular mechanisms that control cell-surface localization of glucose transporters under glucose-limited conditions
Grant-in-Aid for Young Scientists (B)
Kurume University
01 Apr. 2015 - 31 Mar. 2018 - Regulation of the expression and the function of glucose transporters in response to the enviromental glucose concentrations.
Grant-in-Aid for Scientific Research (C)
Kurume University
01 Apr. 2012 - 31 Mar. 2015 - Identification and analysis of biological systems required for efficient glucose consumption
Grant-in-Aid for Young Scientists (B)
Kurume University
2010 - 2011 - Functional analysis of kinetochore proteins by utilizing engineered fission yeast cells harboring a single chromosome.
Grant-in-Aid for Young Scientists (B)
Kurume University
2008 - 2009 - Analysis of the signal network regulating the mitotic checkpoint
Grant-in-Aid for Scientific Research on Priority Areas
Kurume University
2007 - 2008
