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Toyoda Yuusuke
Division of Cell Biology Institute of Life Science
Assistant Professor
Researcher information
■ Research Keyword
■ Research Theme
- Energy metabolism
- Cellular adaptation to glucose-limited environment
- Cell proliferation
- 細胞メカニクス
- RNAi screen
- ゲノミクス
- 細胞分裂
- Cell mechanics
- Genomics
- Mitosis
- Life sciences, Cell biology
- Life sciences, Molecular biology
- Life sciences, Morphology, anatomy
- Life sciences, Genetics
■ Research Theme
Career
■ Career
- Apr. 2022 - Present
Kurume University, Institute of Life Science, Lecturer - Sep. 2014 - Mar. 2022
Kurume University, Institute of Life Science, Division of Cell Biology, Research associate - Apr. 2010 - Aug. 2014
MPI-CBG (Post-doc) - Apr. 2008 - Mar. 2010
- - Apr. 2008 - Mar. 2010
MPI-CBG (JSPS Special Research Fellow Overseas) - Apr. 2007 - Mar. 2008
MPI-CBG (Post-doc) - Apr. 2006 - Mar. 2007
マックスプランク分子細胞生物学遺伝学研究所（上原記念生命科学財団） - Apr. 2006 - Mar. 2007
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) (Uehara Memorial Foundation, post-doc) - Apr. 2005 - Mar. 2006
Kyoto University, Graduate School of Biostudies - Apr. 2005 - Mar. 2006
Kyoto University, Graduate School of Biostudies (Researcher) - Apr. 2003 - Mar. 2005
日本学術振興会特別研究員(D2, PD) - Apr. 2003 - Mar. 2005
JSPS Special Research Fellow (D2, PD)
Research activity information
■ Paper
■ Research Themes
■ 共同研究希望
■ 共同研究実績
- In fission yeast, 65 non-essential mitochondrial proteins related to respiration and stress become essential in low-glucose conditions
Ayaka Mori; Lisa Uehara; Yusuke Toyoda; Fumie Masuda; Saeko Soejima; Shigeaki Saitoh; Mitsuhiro Yanagida
Royal Society Open Science, 18 Oct. 2023 - Fission Yeast TORC2 Signaling Pathway Ensures Cell Proliferation under Glucose-Limited, Nitrogen-Replete Conditions.
Yusuke Toyoda; Shigeaki Saitoh
Biomolecules, 06 Oct. 2021 - TORC2 inhibition of α-arrestin Aly3 mediates cell surface persistence of S. pombe Ght5 glucose transporter in low glucose.
Yusuke Toyoda; Saeko Soejima; Fumie Masuda; Shigeaki Saitoh
Journal of cell science, 15 May 2021 - Multiple nutritional phenotypes of fission yeast mutants defective in genes encoding essential mitochondrial proteins
Lisa Uehara; Shigeaki Saitoh; Ayaka Mori; Kenichi Sajiki; Yusuke Toyoda; Fumie Masuda; Saeko Soejima; Yuria Tahara; Mitsuhiro Yanagida
Open Biology, Apr. 2021 - Extracellular glucose level regulates dependence on GRP78 for cell surface localization of multipass transmembrane proteins in HeLa cells.
Toyoda Y; Akarlar B; Sarov M; Ozlu N; Saitoh S
FEBS letters, Oct. 2018, [Reviewed] - Genome-scale single-cell mechanical phenotyping reveals disease-related genes involved in mitotic rounding
Yusuke Toyoda; Cedric J. Cattin; Martin P. Stewart; Ina Poser; Mirko Theis; Teymuras V. Kurzchalia; Frank Buchholz; Anthony A. Hyman; Daniel J. Mueller
NATURE COMMUNICATIONS, Nov. 2017, [Reviewed] - Rheology of the Active Cell Cortex in Mitosis
Elisabeth Fischer-Friedrich; Yusuke Toyoda; Cedric J. Cattin; Daniel J. Mueller; Anthony A. Hyman; Frank Juelicher
BIOPHYSICAL JOURNAL, Aug. 2016, [Reviewed] - Adaptive regulation of glucose transport, glycolysis and respiration for cell proliferation
Yusuke Toyoda; Shigeaki Saitoh
Biomolecular Concepts, 01 Dec. 2015, [Reviewed] - Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial confinement
Barbara Sorce; Carlos Escobedo; Yusuke Toyoda; Martin P. Stewart; Cedric J. Cattin; Richard Newton; Indranil Banerjee; Alexander Stettler; Botond Roska; Suzanne Eaton; Anthony A. Hyman; Andreas Hierlemann; Daniel J. Mueller
NATURE COMMUNICATIONS, Nov. 2015, [Reviewed] - A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances
Marco Y. Hein; Nina C. Hubner; Ina Poser; Juergen Cox; Nagarjuna Nagaraj; Yusuke Toyoda; Igor A. Gak; Ina Weisswange; Joerg Mansfeld; Frank Buchholz; Anthony A. Hyman; Matthias Mann
CELL, Oct. 2015, [Reviewed] - Quantitative comparison of a human cancer cell surface proteome between interphase and mitosis
Nurhan Ozlu; Mohammad H. Qureshi; Yusuke Toyoda; Bernhard Y. Renard; Gurkan Mollaoglu; Nazli E. Ozkan; Selda Bulbul; Ina Poser; Wiebke Timm; Anthony A. Hyman; Timothy J. Mitchison; Judith A. Steen
EMBO JOURNAL, Jan. 2015, [Reviewed] - Products of the Parkinson's disease-related glyoxalase DJ-1, D-lactate and glycolate, support mitochondrial membrane potential and neuronal survival
Yusuke Toyoda; Cihan Erkut; Francisco Pan-Montojo; Sebastian Boland; Martin P. Stewart; Daniel J. Mueller; Wolfgang Wurst; Anthony A. Hyman; Teymuras V. Kurzchalia
BIOLOGY OPEN, Aug. 2014, [Reviewed] - A Conserved RhoGAP Limits M Phase Contractility and Coordinates with Microtubule Asters to Confine RhoA during Cytokinesis.
Zanin E; Desai A; Poser I; Toyoda Y; Andree C; Moebius C; Bickle M; Conradt B; Piekny A; Oegema K
Developmental cell, Sep. 2013, [Reviewed] - A genomic toolkit to investigate kinesin and myosin motor function in cells
Zoltan Maliga; Magno Junqueira; Yusuke Toyoda; Andreas Ettinger; Felipe Mora-Bermudez; Robin W. Klemm; Andrej Vasilj; Elaine Guhr; Itziar Ibarlucea-Benitez; Ina Poser; Ezio Bonifacio; Wieland B. Huttner; Andrej Shevchenko; Anthony A. Hyman
NATURE CELL BIOLOGY, Mar. 2013, [Reviewed] - C11ORF24 is a novel type I membrane protein that cycles between the Golgi apparatus and the plasma membrane in Rab6-positive vesicles.
Fraisier V; Kasri A; Miserey-Lenkei S; Sibarita JB; Nair D; Mayeux A; Bardin S; Toyoda Y; Poser I, Poznyakovskiy A; Goud B; Hyman AA; Dimitrov A
PloS one, 2013, [Reviewed] - APC15 mediates CDC20 autoubiquitylation by APC/C-MCC and disassembly of the mitotic checkpoint complex
Kristina Uzunova; Billy T. Dye; Hannelore Schutz; Rene Ladurner; Georg Petzold; Yusuke Toyoda; Marc A. Jarvis; Nicholas G. Brown; Ina Poser; Maria Novatchkova; Karl Mechtler; Anthony A. Hyman; Holger Stark; Brenda A. Schulman; Jan-Michael Peters
NATURE STRUCTURAL & MOLECULAR BIOLOGY, Nov. 2012, [Reviewed] - Tracking mechanics and volume of globular cells with atomic force microscopy using a constant-height clamp
Martin P. Stewart; Yusuke Toyoda; Anthony A. Hyman; Daniel J. Mueller
NATURE PROTOCOLS, Jan. 2012, [Reviewed] - Epigenetic displacement of HP1 from heterochromatin by HIV-1 Vpr causes premature sister chromatid separation
Mari Shimura; Yusuke Toyoda; Kenta Iijima; Masanobu Kinomoto; Kenzo Tokunaga; Kinya Yoda; Mitsuhiro Yanagida; Tetsutaro Sata; Yukihito Ishizaka
JOURNAL OF CELL BIOLOGY, Sep. 2011, [Reviewed] - Force probing cell shape changes to molecular resolution
Martin P. Stewart; Yusuke Toyoda; Anthony A. Hyman; Daniel J. Muller
TRENDS IN BIOCHEMICAL SCIENCES, Aug. 2011, [Reviewed] - Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods
Lis Jakobsen; Katja Vanselow; Marie Skogs; Yusuke Toyoda; Emma Lundberg; Ina Poser; Lasse G. Falkenby; Martin Bennetzen; Jens Westendorf; Erich A. Nigg; Mathias Uhlen; Anthony A. Hyman; Jens S. Andersen
EMBO JOURNAL, Apr. 2011, [Reviewed] - Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding
Martin P. Stewart; Jonne Helenius; Yusuke Toyoda; Subramanian P. Ramanathan; Daniel J. Muller; Anthony A. Hyman
NATURE, Jan. 2011, [Reviewed] - Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins
James R. A. Hutchins; Yusuke Toyoda; Bjoern Hegemann; Ina Poser; Jean-Karim Heriche; Martina M. Sykora; Martina Augsburg; Otto Hudecz; Bettina A. Buschhorn; Jutta Bulkescher; Christian Conrad; David Comartin; Alexander Schleiffer; Mihail Sarov; Andrei Pozniakovsky; Mikolaj Michal Slabicki; Siegfried Schloissnig; Ines Steinmacher; Marit Leuschner; Andrea Ssykor; Steffen Lawo; Laurence Pelletier; Holger Stark; Kim Nasmyth; Jan Ellenberg; Richard Durbin; Frank Buchholz; Karl Mechtler; Anthony A. Hyman; Jan-Michael Peters
SCIENCE, Apr. 2010, [Reviewed] - BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals
Ina Poser; Mihail Sarov; James R. A. Hutchins; Jean-Karim Heriche; Yusuke Toyoda; Andrei Pozniakovsky; Daniela Weigl; Anja Nitzsche; Bjoern Hegemann; Alexander W. Bird; Laurence Pelletier; Ralf Kittler; Sujun Hua; Ronald Naumann; Martina Augsburg; Martina M. Sykora; Helmut Hofemeister; Youming Zhang; Kim Nasmyth; Kevin P. White; Steffen Dietzel; Karl Mechtler; Richard Durbin; A. Francis Stewart; Jan-Michael Peters; Frank Buchholz; Anthony A. Hyman
NATURE METHODS, May 2008, [Reviewed] - Coordinated requirements of human Topo II and cohesin for metaphase centromere alignment under Mad2-dependent spindle checkpoint surveillance
Y Toyoda; M Yanagida
MOLECULAR BIOLOGY OF THE CELL, May 2006, [Reviewed] - Requirement of chromatid cohesion proteins rad21/scc1 and mis4/scc2 for normal spindle-kinetochore interaction in fission yeast.
Yusuke Toyoda; Kanji Furuya; Gohta Goshima; Koji Nagao; Kohta Takahashi; Mitsuhiro Yanagida
Current biology : CB, 05 Mar. 2002, [Reviewed]
- AID, which is ectopically expressed in colonic epithelial cells under inflammatory conditions, regulates target gene expression by inducing DNA demethylation.
岡田季之; 豊田雄介; 溝口充志; 齋藤成昭
日本分子生物学会年会プログラム・要旨集(Web), 2023 - Roles of the TORC2 signaling pathway in adaptation to glucose limitation - Molecular mechanisms how amino acids control sugar metabolism
Yusuke Toyoda
Experimental Medicine, Apr. 2022, [Invited] - Identification of disease-related genes required for mitotic cell mechanics
TOYODA Yusuke
Experimental Medicine, Apr. 2018, [Invited] - Measurement of Mitotic Cell Mechanics by Atomic Force Microscopy
TOYODA Yusuke
SEIBUTSU BUTSURI, Apr. 2018, [Reviewed], [Invited] - Mitotic Cell Shape - RNA Interference Screening for Genes Involved in Mechanics using Atomic Force Microscopy
Cedric J. Cattin; Martin P. Stewart; Yusuke Toyoda; Ina Poser; Frank Buchholz; Anthony A. Hyman; Daniel J. Mueller
BIOPHYSICAL JOURNAL, Jan. 2014 - Atomic Force Microscopy to Study Mechanics of Living Mitotic Mammalian Cells
Yusuke Toyoda; Martin P. Stewart; Anthony A. Hyman; Daniel J. Mueller
JAPANESE JOURNAL OF APPLIED PHYSICS, Aug. 2011 - Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins
James R. A. Hutchins; Yusuke Toyoda; Bjoern Hegemann; Ina Poser; Jean-Karim Heriche; Martina M. Sykora; Martina Augsburg; Otto Hudecz; Bettina A. Buschhorn; Jutta Bulkescher; Christian Conrad; David Comartin; Alexander Schleiffer; Mihail Sarov; Andrei Pozniakovsky; Mikolaj Michal Slabicki; Siegfried Schloissnig; Ines Steinmacher; Marit Leuschner; Andrea Ssykor; Steffen Lawo; Laurence Pelletier; Holger Stark; Kim Nasmyth; Jan Ellenberg; Richard Durbin; Frank Buchholz; Karl Mechtler; Anthony A. Hyman; Jan-Michael Peters
SCIENCE, Apr. 2010 - Force Probing the Molecular Mechanics of Cell Rounding
Daniel J. Mueller; Martin P. Stewart; Jonne Helenius; Yusuke Toyoda; Subramaniam P. Rmanathan; Anthony A. Hyman
BIOPHYSICAL JOURNAL, Jan. 2010 - BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals (vol 5, pg 409, 2008)
Ina Poser; Mihail Sarov; James R. A. Hutchins; Jean-Karim Heriche; Yusuke Toyoda; Andrei Pozniakovsky; Daniela Weigl; Anja Nitzsche; Bjoen Hegemann; Alexander W. Bird; Laurence Pelletier; Ralf Kittler; Sujun Hua; Ronald Naumann; Martina Augsburg; Martina M. Sykora; Helmut Hofemeister; Youming Zhang; Kim Nasmyth; Kevin P. White; Steffen Dietzel; Karl Mechtler; Richard Durbin; A. Francis Stewart; Jan-Michael Peters; Frank Buchholz; Anthony A. Hyman
NATURE METHODS, Aug. 2008 - BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals
Ina Poser; Mihail Sarov; James R. A. Hutchins; Jean-Karim Heriche; Yusuke Toyoda; Andrei Pozniakovsky; Daniela Weigl; Anja Nitzsche; Bjoern Hegemann; Alexander W. Bird; Laurence Pelletier; Ralf Kittler; Sujun Hua; Ronald Naumann; Martina Augsburg; Martina M. Sykora; Helmut Hofemeister; Youming Zhang; Kim Nasmyth; Kevin P. White; Steffen Dietzel; Karl Mechtler; Richard Durbin; A. Francis Stewart; Jan-Michael Peters; Frank Buchholz; Anthony A. Hyman
NATURE METHODS, May 2008 - Priming of centromere for CENP-A recruitment by human hMis18 alpha, hMis18 beta, and M18BP1
Yohta Fujita; Takeshi Hayashi; Tomomi Kiyomitsu; Yusuke Toyoda; Aya Kokubu; Chikashi Obuse; Mitsuhiro Yanagida
DEVELOPMENTAL CELL, Jan. 2007 - Coordinated requirements of human Topo II and cohesin for metaphase centromere alignment under Mad2-dependent spindle checkpoint surveillance
Y Toyoda; M Yanagida
MOLECULAR BIOLOGY OF THE CELL, May 2006 - Conserved Mis12/Mtw1-containing centromere complex in fission yeast and HeLa: Link to heterochromatin HP1 and outer kinetochore protein Zwint-1
C Obuse; O Iwasaki; T Kiyomitsu; G Goshima; Y Toyoda; M Yanagida
MOLECULAR BIOLOGY OF THE CELL, Nov. 2004 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation
T Yuasa; T Hayashi; N Ikai; T Katayama; K Aoki; T Obara; Y Toyoda; T Maruyama; D Kitagawa; K Takahashi; K Nagao; Y Nakaseko; M Yanagida
GENES TO CELLS, Nov. 2004 - A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1
C Obuse; O Iwasaki; T Kiyomitsu; G Goshima; Y Toyoda; M Yanagida
NATURE CELL BIOLOGY, Nov. 2004 - Requirement of chromatid cohesion proteins Rad21/Scc1 and Mis4/Scc2 for normal spindle-kinetochore interaction in fission yeast
Y Toyoda; K Furuya; G Goshima; K Nagao; K Takahashi; M Yanagida
CURRENT BIOLOGY, Mar. 2002
- Phosphorylation of the carboxyl terminus of the S. pombe arrestin Aly3 suppresses endocytosis of the hexose transporter Ght5
Yusuke Toyoda
The 47th Annual Meeting of the Molecular Biology Society of Japan, 29 Nov. 2024 - TORC2 inhibits α-arrestin Aly3-dependent vacuolar transport of the S. pombe hexose transporter Ght5
Yusuke Toyoda
The 44th Annual Meeting of the Molecular Biology Society of Japan (MBSJ2021), 03 Dec. 2021 - Nitrogen-dependent persistence of S. pombe Ght5 glucose transporter on the cell surface is mediated by TORC2 inhibition of α-arrestin Aly3
Yusuke Toyoda
pombeTalks S02 E07, 09 Jun. 2021, [Invited] - パーキンソン病関連グリオキサラーゼDJ-1の 原子間力顕微鏡を用いた生物物理学的研究
豊田雄介
第48回分子医学入門塾, 17 May 2016, [Invited] - Mechanisms how Parkinson’s disease-related glyoxalase DJ-1 supports mitochondrial membrane potential and in vitro survival of dopaminergic neurons.
TOYODA Yusuke
Kurume University, Institute of Life Science, 24 Apr. 2014, [Invited] - Mechanisms how Parkinson’s disease-related glyoxalase DJ-1 supports mitochondrial membrane potential and in vitro survival of dopaminergic neurons.
TOYODA Yusuke
OIST Seminar, 22 Apr. 2014, [Invited] - Genetic analysis of mitotic mammalian cell mechanics.
TOYODA Yusuke
138th iCeMS Seminar, 25 Jun. 2013, [Invited] - Opposing activities of hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding.
TOYODA Yusuke
18th International Colloquium on Scanning Probe Microscopy (ICSPM18), 09 Dec. 2010, [Invited] - Human mitotic proteome - localization- and interaction-based approaches to study protein functions in mitosis
第32回日本分子生物学会年会, 2009 - Localization-based functional screening of the human mitotic genes isolated in a genome-wide RNAi screen
BMB 2007 (第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会), 2007 - Localization-based functional screening of the human mitotic genes isolated in a genome-wide RNAi screen
ELSO 2007 (European Life Scientist Organization), 2007
■ Research Themes
- Molecular mechanisms how arrestins that modulate localization of glucose transporters are phosphorylated in response to amino acids
Grant-in-Aid for Scientific Research (C)
Kurume University
Apr. 2023 - Mar. 2026 - The role of two TOR complexes in adaptation of eukaryotic cells to the low-glucose environment
Grant-in-Aid for Scientific Research (C)
Kurume University
01 Apr. 2020 - 31 Mar. 2023 - Molecular mechanisms that control cell-surface localization of glucose transporters under glucose-limited conditions
Grant-in-Aid for Young Scientists (B)
Kurume University
01 Apr. 2015 - 31 Mar. 2018 - 細胞分裂に必要なヒトタンパク質の細胞内局在クラスタリングによる、遺伝子機能の予測および解析
2007 - 2010 - Prediction and analysis of functions of the human mitotic proteins through sub-cellular localization-based clustering
2006 - 2010 - ヒトタンパク質hMis4, hRad21の、RNA干渉法を用いた機能的解析
2003 - Functional analysis of human hMis4 and hRad21 by RNA interference
2003 - Functional analysis of Mis4, essential for sister chromatid cohesion
- 特願EP14162874, Glycolic acid and/or D-lactic acid for the treatment of neurodegenerative diseases.
TOYODA Yusuke; ERKUT Cihan; PAN-MONTOJO Francisco; HYMAN Anthony; KURZCHALIA Teymuras
■ 共同研究希望
■ 共同研究実績
- Dr. Nurhan Ozluグループ(トルコ, Koc大学)との共同研究の結果が公表されました(Toyoda et al, FEBS Letters, 2018)。
- 柳田充弘教授グループ(沖縄科学技術大学院大学 G0研究ユニット)との共同研究の結果が公表されました(Uehara et al, Open Biology, 2021)。
